Plague Research Today is a free monthly online journal that collates and summarizes the latest research about Plague, including details on bubonic plague, yersinia pestis, infection, types, treatment.

Identification of outer membrane proteins of Yersinia pestis through biotinylation.

Smither SJ, Hill J, van Baar BL, Hulst AG, de Jong AL, Titball RW

Dstl, Porton Down, Room 201/Bldg 7a, Salisbury, WILTS, SP4 OJQ, UK.

The outer membrane of Gram-negative bacteria contains proteins that might be good targets for vaccines, antimicrobials or detection systems. The identification of surface located proteins using traditional methods is often difficult. Yersinia pestis, the causative agent of plague, was labelled with biotin. Tagged proteins were visualised through streptavidin probing of Western blots. Seven biotinylated proteins of Y. pestis were identified including two porins and the putative virulence factor catalase peroxidase.

Published 15 January 2007 in J Microbiol Methods, 68(1): 26-31.
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